normal human colon mucosal epithelial cell line ncm460  (European Collection of Authenticated Cell Cultures)

Journal: Oncotarget

Article Title: Twist1 confers multidrug resistance in colon cancer through upregulation of ATP-binding cassette transporters

doi: 10.18632/oncotarget.17548

Figure Lengend Snippet: (A) IC 50 values of HCT-8/V and parental HCT-8 cells for vincristine, taxol, and cisplatin; RI represents the resistance index. (B) Rh123 efflux was measured using flow cytometry. (C) Western blot analysis of ABCB1 and ABCC1; GAPDH was used as a loading control. (D) Increased actin microfilament was observed in the HCT-8/V cells by cytoskeleton staining. (E) Immunofluorescence staining of epithelial and mesenchymal markers in HCT-8 and HCT-8/V cells. Cells were double-stained for vimentin (red) and E-cadherin (green). Nuclei were counterstained blue with DAPI. (F) Expression of EMT markers and transcription factors was determined by Western blot. GAPDH was used as a loading control. Scale bars represent 10 μm in D and E . Data from three independent experiments were graphed as the mean ± SD. * P < 0.05.

Article Snippet: The normal human colon mucosal epithelial cell line (NCM460), the low invasive human colon adenocarcinoma cell line (HT-29), the highly invasive human colon cancer cells (HCT-8), HCT-8/V and Bel7402 hepatocarcinoma cell line were purchased from KeyGEN BioTECH (Nanjing, China).

Techniques: Flow Cytometry, Western Blot, Control, Staining, Immunofluorescence, Expressing